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Developed using probability state modeling, a proven high-parameter analytic approach, the software eliminates the variability of manual gating and provides an efficient and reliable solution for data analysis in longitudinal and multisite studies. Cytobank cloud-based data analysis software turns high-content data into high-impact knowledge by providing tools optimized for mass cytometry, including clustering SPADE and viSNE , heat maps, dot plots, histograms and statistics, along with the ability to share your data with colleagues and collaborators.

Stain Stain cells using Fluidigm-validated protocols, buffers and barcoding reagents.

Acquire Load prepared cells into Helios, which ionizes and releases the metal tags from individual cells, separates them by mass and counts the number bound to each cell. Analyze View and analyze high-dimensional data sets through a convenient cloud-based platform, Cytobank. Maxpar Direct Assays Easily quantify 37 immune cell populations using a simple single-tube workflow with automated 5-minute results reporting. Maxpar Metal Labeling Kits Conjugate metals to antibodies or other proteins in as little as two and a half hours with our Maxpar labeling kits , optimized for use with CyTOF systems.

Cell-ID Plex Pd Barcoding Kit Improve data quality, increase throughput and collect more exciting data with a set of unique palladium barcoding reagents and advanced debarcoding software. Maxpar Sample Prep Buffers Optimize your mass cytometry staining with our sample prep buffers , which can be used in surface, intracellular, nuclear and DNA staining protocols.

Maxpar Metal-Conjugated Antibodies Achieve comprehensive functional and phenotypic profiling of cell samples with antibodies validated for functionality and compatibility.

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Flow Cytometry: Principles and Clinical Applications in Hematology

Maxpar Panel Kits Analyze a variety of proteins in cell populations with our ready-to-use kits containing up to 17 metal-conjugated antibodies. Areas of inquiry include stem-cell development, cell cycle, signaling and immunology. Fluidigm Cytobank Analysis Cytobank cloud-based data analysis software turns high-content data into high-impact knowledge by providing tools optimized for mass cytometry, including clustering SPADE and viSNE , heat maps, dot plots, histograms and statistics, along with the ability to share your data with colleagues and collaborators.

Helios System Channels Mass range 75— amu Abundance sensitivity 0.

Multicolor Analysis of Cell Surface Marker of Human Leukemia Cell Lines Using Flow Cytometry

Comprehensive Accelerate insight into mechanisms of health and disease with the most comprehensive view of cell phenotype and function. Refresh and try again. Open Preview See a Problem? Details if other :. Thanks for telling us about the problem. Return to Book Page.

General Principles

McCarthy Editor ,. Marion G. Macey Editor. Flow cytometry and laser scanning cytometry are increasingly used in clinical and research settings due to improvements in instrument design and computing power and the increased availability of fluorescent agents.

Immunophenotyping of B Cells by Flow Cytometry | Bio-Rad

This book provides a comprehensive introduction to the theory and clinical applications of these techniques in the assessment of cell phenotype and function. Wi Flow cytometry and laser scanning cytometry are increasingly used in clinical and research settings due to improvements in instrument design and computing power and the increased availability of fluorescent agents.

With an emphasis on clinical relevance, the book presents the principles and potential of cytometry in the analysis of phenomena including cell-mediated cytotoxicity, metabolic burst, phagocytosis, cell-cell aggregation, receptor shedding, and apoptosis. The volume guides the reader through data interpretation, quality control procedures, pitfalls, and problems. Get A Copy. Hardcover , pages.

Published November 1st by Cambridge University Press. More Details Original Title.

Materials and Methods

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